In contrast to many classical autotransporters, where a proximal folding core in the passenger is required for secretion, the D00 domain is dispensable, as the passenger of an intimin mutant lacking D00 entirely is efficiently exported. Furthermore, the D00 domain is slightly less stable than the D0 and D1 domains, unfolding at 200 piconewtons (pN) compared with 250 pN for D0 and D1 domains as measured by atomic force microscopy. Our results support a model where the secretion of the passenger is driven by sequential folding of the extracellular Ig like domains, leading to vectorial transport of the passenger domain across the outer membrane in an N to C direction..
Currently the brick storm sewer varies in depth from approximately 9.25 ‘ to an estimated depth of approximately 20’ at the point of failure. Repair of the storm sewer at this location would be exceedingly difficult, instead City Engineering will be rerouting the pipe to an existing drop shaft constructed as part of the retaining wall. This drop shaft will convey the water down and into the existing storm sewer at the base of the retaining wall.
A big jump in turnout of 18 29 year old voters across the country. On the local level, Tenney Lapham’s Ward 45 had more than 100% turnout due to all the new voters! While the legislative balance of power did not change in our state, the election of Tony Evers to Governor will create opportunities to change the leadership and direction of state agencies and revamp budget priorities. Many of you worked hard to make sure every voter voted! As a result, we have a new governor, the first African American Lt Governor Mandela Barnes, Josh Kaul is the new attorney general and Tammy Baldwin was re elected to the US Senate.
The week of February 20 has items of possible interest to residents of district 2 at the , the , the , the Urban Design Commission, the Vending Oversight Committee, the Pedestrian/Bicycle/Motor Vehicle Commission and the Housing Strategy Committee. The city meeting agenda items are open for testimony from the public should you be interested in weighing in. I also appreciate hearing from you on any items on which you have a particular interest or concern so please send me an email or give me a call..
For this episode, I think Croc and Seahorse would win their matchups and give us Mushroom v Jellyfish in the Smackdown. For some reason, Mushroom was put in the bottom over Jellyfish so either they autotuned him to hell or the audience just weren’t feeling him. Either case, I’m putting him in the bottom.